Lack of deviation from Michaelis–Menten kinetics for pig heart fumarase
نویسندگان
چکیده
منابع مشابه
Affinity chromatography of pig heart fumarase.
2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by s...
متن کاملThe Preparation and Characterization of Fumarase from Swine Heart Muscle.
Detailed kinetic studies of the action of crystalline fumarase (fumarate hydratase, EC 4.2.1.2) have been made by Alberty and co-workers (2, 3). These studies have given considerable insight into the mechanism of action of the enzyme and have provided a basis for understanding one type of enzymically catalyzed hydration reaction. At present, however, little is known of those structural features...
متن کاملPig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.
The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic ...
متن کاملFrom Guinea Pig Heart
Effects ofATP on junctional conductance (gj) were investigated in paired ventricular myocytes isolated from guinea pig hearts. One cell of the pair was voltage-clamped with a single-patch pipette, and gj was measured after the perforation of the nonjunctional membrane of the partner cell. The current-voltage relation of gj was linear between -30 and +30 mV. The control gj at 5.0 mM ATP in 88 pa...
متن کاملEffect of Aqueous-Ethanolic Extract from Rosa damascena on Guinea Pig Isolated Heart
Objective(s) In the present study, the effects of aqueous-ethanolic extract from Rosa damascena on heart rate and contractility were examined. Materials and Methods Isolated guinea-pig hearts were perfused through aorta in a Langendorff mode. Heart rate (HR) and contractility were determined in the presence of four concentrations of the extract (0.1, 0.2, 0.4 and 1.0 mg %) and isoprenaline (...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1890653